Hybridization of Variants of Chloramphenicol Acetyltransferase Specified by fi+ and fi- R Factors
نویسندگان
چکیده
منابع مشابه
Identification of "buried" lysine residues in two variants of chloramphenicol acetyltransferase specified by R-factors.
Two variants of chloramphenicol acetyltransferase which are specified by genes on plasmids found in Gram-negative bacteria were subjected to amidination with methyl acetimidate to determine the relative reactivity of surface lysine residues and to search for unreactive or "buried" amino groups which might contribute to stabilization of the native tetramers. Representative examples of the type-I...
متن کاملCovert R Factors in fi+ R+ Strains of Bacteria
Many bacterial characters, such as colicinogeny (9), antibiotic resistance (25), and production of a hemolysin (21) or an enterotoxin (22), are determined by genes carried on extrachromosomal genetic elements that are transmissible from one bacterium to another by conjugation. Two classes of sex factor responsible for conjugation have been recognized in the Enterobacteriaceae (11). The first, o...
متن کاملChloramphenicol Acetyltransferase Variants in Staphylococci
Four electrophoretic variants of chloramphenicol acetyltransferase (types A, B, C and D) found in chloramphenicol-resistant staphylococci were purified by affinity chromatography. Michaelis constants and the kinetics of inactivation with a variety of reagents for the four variants are virtually identical. Their similar amino acid compositions and near identical N-terminal sequences suggest a hi...
متن کاملمدل سازی نوسانات بازده بورس اوراق بهادار تهران مدل MRS-FI-TGARCH و FI-TGARCH
هدف این مقاله افزایش انعطاف پذیری مدل سازی نوسانات بازار سرمایه می باشد. این امربا معرفی مدل MRS-FI-TGARCH برای اولین بار در دنیا انجام می گیرد. به این منظور از شاخص هفتگی قیمت بورس اوراق بهادار تهران طی سالهای ۲۰۰۹ تا ۲۰۱۷ استفاده می شود .پارامترها قابلیت تغییر با رژیم را دارند. نتایج نشان داد دو رژیم رونق، با بازده انتظاری بالا و نوسان بالا و رژیم رکود، با بازده انتظاری پایین و نوسان پایینوجو...
متن کاملCharacterization of the chloramphenicol acetyltransferase variants encoded by the plasmids pSCS6 and pSCS7 from Staphylococcus aureus.
The two 4.6 kb chloramphenicol resistance (CmR) plasmids pSCS6 and pSCS7, previously identified in Staphylococcus aureus from subclinical bovine mastitis, both encoded an inducible chloramphenicol acetyltransferase (CAT, EC 2.3.1.28). The pSCS6- and pSCS7-encoded CAT variants were purified by ammonium sulphate precipitation, ion-exchange chromatography and fast protein liquid chromatography (FP...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1972
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.69.10.3049